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KMID : 0387719920030020159
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Korean Journal of Blood Transfusion
1992 Volume.3 No. 2 p.159 ~ p.165
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Comparison of Three Methods for Inactivation IgM Antibodies for Determination of IgG Anti-A or anti-B-Dithiotreitol, 2-Mercaptoethanol, NeutrAB-
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김현옥
조현석/박광수/권오헌/김진주
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Abstract
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Three methods were evaluated for their inactivation capability of IgM anti-A and IgM anti-B in sera: 1) 0.01 M dithiothreitol (DTT) with equal volume of serum was incubated for 2 hours at 37℃, 2) 0.2 M 2-mercaptoethanol (2-ME) with equal volume
of
serum was incubated for 1 hour at 37℃, and 3) neutr AB(r) with 5 volume of serum was incubated for 15 minutes at room temperature. Thirty samples of group O blood were randomly selected and tested by these method. For IgG anti-A and IgG anti-B
titration, indirect antiglobulin method was used.
1. After the inactivation of IgM anti-A and IgM anti-B by the methods, the titers ranged from 1:16 to 1:2048 and from 1:16 to 1:1024 for IgG anti-A and IgG anti-B respectively. The median values were all same in the three methods (1:128 for IgG
anti-A
and 1:64 for IgG anti-B).
2. The concordance of the IgG anti-A between two methods was 87% for the DTT method and the 2-ME method, and 80% for the DTT method and the NeutrAB(r) method and 77% for the 2-ME and the NeutrAB(r) method. The concordance of the IgG anti-B
between
two
methods 93% for the DTT method and the 2-ME method, 87% for the DTT method and the NeutrAB(r) method and 77% for the 2-ME method and the NeutrAB(r). Except for the concordance between the NeutrAB(r) method and the 2-ME method, the concordances of
other
combinations were insignificant statistically (P>0.05).
3. The titers of IgM anti-A and IgM anti-B ranged from 1:32 to 1:4096 (median 1:256, mean 1:564) and 1:32 to 1:2048 (median 1:128, mean 1:289), respecitively. The sera with higher titer of IgG than of IgM were observed in 7~46%
Though there wero no significant differences among the three methods for the inactivation of IgM anti-A and anti-B in the serum, the DTT method was considered as a resonable method to use in a clinical laboratory, and no matter which method is
used,
strict and precise performance of the procedure is the most important factor to get good results.
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